Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: S100A8 and S100A9 in experimental osteoarthritis. | Zreiqat et al. Arthritis Research Therapy 2010 12 R16 http content 12 1 R16 RESEARCH ARTICLE Open Access S100A8 and S100A9 in experimental osteoarthritis I_I -A I -A 7 I z t s 1 f h I ỉ I f Dz- .lli I fs r r I f 2 l IV ỉ vr r IV I c rv I 3 D I r r I A ỉ I r r V 2 I I Ti DcmaJ v 2 I f 7 X r 1 Hala zreiqat Daniele Belluoccio Margaret M Smith Richard Wilson Lynn A Rowley Katie Jones z-s. S-J s IZV r S D s m s ft A s V VW r I S r Ịf z t 14 I A J r D I 4 I A c D J ws 2 f I r I ỉ J K D I I I J 3 vogambha Ramaswamy Thomas vogi Johannes noth Jonn F Bateman Christopher B Little Abstract Introduction The objective was to evaluate the changes in S100A8 S100A9 and their complex S100A8 S100A9 in cartilage during the onset of osteoarthritis OA as opposed to inflammatory arthritis. Methods S100A8 and S100A9 protein localization were determined in antigen-induced inflammatory arthritis in mice mouse femoral head cartilage explants stimulated with interleukin-1 IL-1 and in surgically-induced OA in mice. Microarray expression profiling of all S100 proteins in cartilage was evaluated at different times after initiation of degradation in femoral head explant cultures stimulated with IL-1 and surgically-induced OA. ihe effect of S100A8 S100A9 or the complex on the expression of aggrecan Acan collagen II Col2a1 disintegrin and metalloproteases with thrombospondin motifs Adamtsl Adamts 4 Adamts 5 matrix metalloproteases Mmp1 Mmp3 Mmp13 Mmp14 and tissue inhibitors of metalloproteinases Timpl Timp2 Timp3 by primary adult ovine articular chondrocytes was determined using real time quantitative reverse transcription polymerase chain reaction qRT-PCR . Results Stimulation with IL-1 increased chondrocyte S100a8 and S100a9 mRNA and protein levels. There was increased chondrocyte mRNA expression of S100a8 and S100a9 in early but not late mouse OA. However loss of the S100A8 staining in chondrocytes occurred as mouse OA progressed in contrast to the positive reactivity for
