Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Mitochondrial dysfunction and mitophagy activation in blood mononuclear cells of fibromyalgia patients: implications in the pathogenesis of the disease. | Cordero et al. Arthritis Research Therapy 2010 12 R17 http content 12 1 R17 RESEARCH ARTICLE Open Access Mitochondrial dysfunction and mitophagy activation in blood mononuclear cells of fibromyalgia patients implications in the pathogenesis of the disease Mario D Cordero21 2 3 Manuel De Miguel23 Ana M Moreno Fernandez3 Inés M Carmona Lopez3 Juan Garrido Maraver 1 2 David Cotán1 2 Lourdes Gomez Izquierdo4 Pablo Bonal5 Francisco Campa5 6 Pedro Bullon7 Plácido Navas1 2 and José A Sanchez Alcazar 1 2 Abstract Introduction Fibromyalgia is a chronic pain syndrome with unknown etiology. Recent studies have shown some evidence demonstrating that oxidative stress may have a role in the pathophysiology of fibromyalgia. However it is still not clear whether oxidative stress is the cause or the effect of the abnormalities documented in fibromyalgia. Furthermore the role of mitochondria in the redox imbalance reported in fibromyalgia also is controversial. We undertook this study to investigate the role of mitochondrial dysfunction oxidative stress and mitophagy in fibromyalgia. Methods We studied 20 patients 2 male 18 female patients from the database of the Sevillian Fibromyalgia Association and 10 healthy controls. We evaluated mitochondrial function in blood mononuclear cells from fibromyalgia patients measuring coenzyme Q10 levels with high-performance liquid chromatography HPLC and mitochondrial membrane potential with flow cytometry. Oxidative stress was determined by measuring mitochondrial superoxide production with MitoSOX and lipid peroxidation in blood mononuclear cells and plasma from fibromyalgia patients. Autophagy activation was evaluated by quantifying the fluorescence intensity of LysoTracker Red staining of blood mononuclear cells. Mitophagy was confirmed by measuring citrate synthase activity and electron microscopy examination of blood mononuclear cells. Results We found reduced levels of coenzyme Q10 decreased mitochondrial membrane
