Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Smoking-mediated up-regulation of GAD67 expression in the human airway epithelium. | Wang et al. Respiratory Research 2010 11 150 http content 11 1 150 RESPIRATORY RESEARCH RESEARCH Open Access Smoking-mediated up-regulation of GAD67 expression in the human airway epithelium Guoqing Wang Rui Wang Barbara Ferris Jacqueline Salit Yael Strulovici-Barel Neil R Hackett Ronald G Crystal Abstract Background The production of gamma-amino butyric acid GABA is dependent on glutamate decarboxylases GAD65 and GAD67 the enzymes that catalyze the decarboxylation of glutamate to GABA. Based on studies suggesting a role of the airway epithelial GABAergic system in asthma-related mucus overproduction we hypothesized that cigarette smoking another disorder associated with increased mucus production may modulate GABAergic system-related gene expression levels in the airway epithelium. Methods We assessed expression of the GABAergic system in human airway epithelium obtained using bronchoscopy to sample the epithelium and microarrays to evaluate gene expression. RT-PCR was used to confirm gene expression of GABAergic system gene in large and small airway epithelium from heathy nonsmokers and healthy smokers. The differences in the GABAergic system gene was further confirmed by TaqMan immunohistochemistry and Western analysis. Results The data demonstrate there is a complete GABAergic system expressed in the large and small human airway epithelium including glutamate decarboxylase GABA receptors transporters and catabolism enzymes. Interestingly of the entire GABAergic system smoking modified only the expression of GAD67 with marked upregulation of GAD67 gene expression in both large increase p and small airway epithelium of healthy smokers increase p . At the protein level Western analysis confirmed the increased expression of GAD67 in airway epithelium of healthy smokers compared to healthy nonsmokers p . There was a significant positive correlation between GAD67 and MUC5AC gene expression in both large and .
