Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Inhibitors of inflammation and endogenous surfactant pool size as modulators of lung injury with initiation of ventilation in preterm sheep. | Hillman et al. Respiratory Research 2010 11 151 http content 11 1 151 RESPIRATORY RESEARCH RESEARCH Open Access Inhibitors of inflammation and endogenous surfactant pool size as modulators of lung injury with initiation of ventilation in preterm sheep 1 2 2 2 1 Noah H Hillman Suhas G Kallapur J Jane Pillow Ilias Nitsos Graeme R Polglase Machiko Ikegami Alan H Jobe1 2 Abstract Background Increased pro-inflammatory cytokines in tracheal aspirates correlate with the development of BPD in preterm infants. Ventilation of preterm lambs increases pro-inflammatory cytokines and causes lung inflammation. Objective We tested the hypothesis that selective inhibitors of pro-inflammatory signaling would decrease lung inflammation induced by ventilation in preterm newborn lambs. We also examined if the variability in injury response was explained by variations in the endogenous surfactant pool size. Methods Date-mated preterm lambs n 28 were operatively delivered and mechanically ventilated to cause lung injury tidal volume escalation to 15 mL kg by 15 min at age . The lambs then were ventilated with 8 mL kg tidal volume for 1 h 45 min. Groups of animals randomly received specific inhibitors for IL-8 IL-1 or NF-kB. Unventilated lambs n 7 were the controls. Bronchoalveolar lavage fluid BALF and lung samples were used to quantify inflammation. Saturated phosphatidylcholine Sat PC was measured in BALF fluid and the data were stratified based on a level of 5 gmol kg 8 mg kg surfactant . Results The inhibitors did not decrease the cytokine levels or inflammatory response. The inflammation increased as Sat PC pool size in BALF decreased. Ventilated lambs with a Sat PC level 5 gmol kg had significantly decreased markers of injury and lung inflammation compared with those lambs with 5 gmol kg. Conclusion Lung injury caused by high tidal volumes at birth were decreased when endogenous surfactant pool sizes were larger. Attempts to decrease inflammation by .
