Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:The effect of interleukin-13 (IL-13) and interferon-g (IFN-g) on expression of surfactant proteins in adult human alveolar type II cells in vitro. | Ito and Mason Respiratory Research 2010 11 157 http content 11 1 157 RESPIRATORY RESEARCH RESEARCH Open Access The effect of interleukin-13 IL-13 and interferon-g IFN-g on expression of surfactant proteins in adult human alveolar type II cells in vitro Yoko Ito Robert J Mason Abstract Background Surfactant proteins are produced predominantly by alveolar type II ATII cells and the expression of these proteins can be altered by cytokines and growth factors. Th1 Th2 cytokine imbalance is suggested to be important in the pathogenesis of several adult lung diseases. Recently we developed a culture system for maintaining differentiated adult human ATII cells. Therefore we sought to determine the effects of IL-13 and IFN-g on the expression of surfactant proteins in adult human ATII cells in vitro. Additional studies were done with rat ATII cells. Methods Adult human ATII cells were isolated from deidentified organ donors whose lungs were not suitable for transplantation and donated for medical research. The cells were cultured on a mixture of Matrigel and rat-tail collagen for 8 d with differentiation factors and human recombinant IL-13 or IFN-g. Results IL-13 reduced the mRNA and protein levels of surfactant protein SP -C whereas IFN-g increased the mRNA level of SP-C and proSP-C protein but not mature SP-C. Neither cytokine changed the mRNA level of SP-B but IFN-g slightly decreased mature SP-B. IFN-g reduced the level of the active form of cathepsin H. IL-13 also reduced the mRNA and protein levels of SP-D whereas IFN-g increased both mRNA and protein levels of SP-D. IL-13 did not alter SP-A but IFN-g slightly increased the mRNA levels of SP-A. Conclusions We demonstrated that IL-13 and IFN-g altered the expression of surfactant proteins in human adult ATII cells in vitro. IL-13 decreased SP-C and SP-D in human ATII cells whereas IFN-g had the opposite effect. The protein levels of mature SP-B were decreased by IFN-g treatment likely due to .
