Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Oral tolerance inhibits pulmonary eosinophilia in a cockroach allergen induced model of asthma: a randomized laboratory study. | Vaickus et al. Respiratory Research 2010 11 160 http content 11 1 160 RESPIRATORY RESEARCH RESEARCH Open Access Oral tolerance inhibits pulmonary eosinophilia in a cockroach allergen induced model of asthma a randomized laboratory study Louis J Vaickus Jacqueline Bouchard Jiyoun Kim Sudha Natarajan Daniel G Remick Abstract Background Antigen desensitization through oral tolerance is becoming an increasingly attractive treatment option for allergic diseases. However the mechanism s by which tolerization is achieved remain poorly defined. In this study we endeavored to induce oral tolerance to cockroach allergen CRA a complex mixture of insect components in order to ameliorate asthma-like allergic pulmonary inflammation. Methods We compared the pulmonary inflammation of mice which had received four CRA feedings prior to intratracheal allergen sensitization and challenge to mice fed PBS on the same time course. Respiratory parameters were assessed by whole body unrestrained plethysmography and mechanical ventilation with forced oscillation. Bronchoalveolar lavage fluid BAL and lung homogenate LH were assessed for cytokines and chemokines by ELISA. BAL inflammatory cells were also collected and examined by light microscopy. Results CRA feeding prior to allergen sensitization and challenge led to a significant improvement in respiratory health. Airways hyperreactivity measured indirectly via enhanced pause Penh was meaningfully reduced in the CRA-fed mice compared to the PBS fed mice vs p . Directly measured airways resistance confirmed this trend when comparing the CRA-fed to the PBS-fed animals vs . This effect was not due to reduced traditional inflammatory cell chemotactic factors Th2 or other cytokines and chemokines. The mechanism of improved respiratory health in the tolerized mice was due to significantly reduced eosinophil numbers in the bronchoalveolar lavage fluid 43300 11445 vs 158786 38908 p
