Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Dysregulated balance of Th17 and Th1 cells in systemic lupus erythematosus. | Shah et al. Arthritis Research Therapy 2010 12 R53 http content 12 2 R53 RESEARCH ARTICLE Open Access Dysregulated balance of Th17 and Th1 cells in systemic lupus erythematosus Kamini Shah1 Won-Woo Lee1 2 Seung-Hyun Lee1 3 Sang Hyun Kim1 4 Seong Wook Kang1 5 Joe Craft1 63 Insoo Kang1 3 Abstract Introduction Interleukin IL -17 is a proinflammatory cytokine that is produced largely by a unique CD4 T-helper Th subset called Th17 cells. The development of Th17 cells is suppressed by interferon IFN -y produced by Th1 cells suggesting cross-regulation between Th17 and Th1 cells. Thus this study analyzed the balance of CD4 Th17 and Th1 cell responses in peripheral blood from patients with systemic lupus erythematosus SLE and healthy subjects. Methods Twenty-five adult patients with SLE and 26 healthy subjects matched for gender and age 2 years were recruited. Peripheral blood mononuclear cells PBMCs from patients and healthy subjects were stimulated for 4 h ex vivo with phorbol myristate acetate PMA and ionomycin. The frequency of CD4 T cells producing IL-17 and or IFN-y was measured by using flow cytometry. Expression of Th17-associated chemokine receptors CCR4 and CCR6 on CD4 T cells as well as plasma levels of Th17-polarizing cytokines were assessed. Disease activity was evaluated by the SLE disease activity index score SLEDAI . Unpaired t test and Pearson correlation were used for statistical analyses. Results Patients with SLE had an increased frequency of CD4 IL-17 T cells compared with healthy subjects. However the frequency of CD4 IFN-g T cells was similar between the two groups indicating an altered balance of Th17 and Th1 cell responses in SLE. Patients with SLE also had an increased frequency of CD4 CCR4 CCR6 T cells that are known to produce IL-17. The frequency of CD4 IL-17 T cells and CD4 CCR4 CCR6 T cells correlated with disease activity. In measuring plasma levels of the Th17-polarizing cytokines levels of IL-6 were higher in .
