Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Elevated expression of both mRNA and protein levels of IL-17A in sputum of stable Cystic Fibrosis patients. | Decraene et al. Respiratory Research 2010 11 177 http content 11 1 177 RESPIRATORY RESEARCH RESEARCH Open Access Elevated expression of both mRNA and protein levels of IL-17A in sputum of stable Cystic Fibrosis patients 1 12 3 Ann Decraene Anna Willems-Widyastuti Ahmad Kasran Kris De Boeck Dominique M Bullens Lieven J Dupont 4 Abstract Background T helper 17 Th17 cells can recruit neutrophils to inflammatory sites through production of IL-17 which induces chemokine release. IL-23 is an important inducer of IL-17 and IL-22 production. Our aim was to study the role of Th17 cells in cystic fibrosis CF lung disease by measuring IL-17 protein and mRNA levels and IL-22 and IL-23 mRNA in sputum of clinically stable CF patients and by comparing these levels with healthy controls. Methods Sputum induction was performed in adult CF patients outside of an exacerbation and healthy control subjects. IL-17A protein levels were measured in supernatants with cytometric bead array CBA and RNA was isolated and quantitative RT-PCR was performed for IL-17A IL-22 and IL-23. Results We found significantly higher levels of IL-17A protein and mRNA levels both p and IL-23 mRNA levels p in the sputum of CF group as compared to controls. We found very low levels of IL-22 mRNA in the CF group. The levels of IL-17 and IL-23 mRNA were higher in patients chronically infected with Pseudomonas aeruginosa P. aeruginosa as compared to those who were not chronically infected with P. aeruginosa. The presence of Staphylococcus aureus S. aureus on sputum did not affect the IL-17 or IL-23 levels. There was no correlation between IL-17 or IL-23 levels and FEV1 nor sputum neutrophilia. Conclusion The elevated levels of IL-17 and IL-23 might indicate that Th17 cells are implicated in the persistent neutrophil infiltration in CF lung disease and chronic infection with P. aeruginosa. Background The major cause of morbidity and mortality in cystic fibrosis CF is lung
