Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:" Mometasone and desloratadine additive effect on eosinophil survival and cytokine secretion from epithelial cells. | Mullol et al. Respiratory Research 2011 12 23 http content 12 1 23 RESPIRATORY RESEARCH RESEARCH Open Access Mometasone and desloratadine additive effect on eosinophil survival and cytokine secretion from epithelial cells 1 1 1 Joaquim Mullol Francisco de Borja Callejas Maria Asuncion Martínez-Antón Eva Mendez-Arancibia 2 1 3 1 Isam Alobid Laura Pujols Antonio Valero Cesar Picado Jordi Roca-Ferrer Abstract Background Although antihistamines and topical corticosteroids are used in combination to treat allergic rhinitis their additive effect has not been yet demonstrated. The aim was investigate the antiinflammatory additive effect of mometasone and desloratadine on cytokine and sICAM-1 secretion by epithelial cells and on eosinophil survival stimulated by human epithelial cells secretions from nasal mucosa and polyps. Methods Epithelial cells obtained from nasal mucosa or polyps were stimulated with 10 fetal bovine serum in presence of mometasone 10-11M-10-5M with without desloratadine 10-5M . Cytokine and sICAM-1 concentrations in supernatants were measured by ELISA. Peripheral blood eosinophils were incubated during 4 days with epithelial cell secretions with 10-11M-10-5M and or desloratadine 10-5M and survival assessed by Trypan blue. Results are expressed as percentage mean SEM compared to control. Results Fetal bovine serum stimulated IL-6 IL-8 GM-CSF and sICAM-1 secretion. In mucosa and polyp epithelial cells mometasone inhibited this induced secretion while desloratadine inhibited IL-6 and IL-8. The combination of 10-5M desloratadine and 10-9M mometasone reduced IL-6 secretion 48 11 p greater extent than mometasone alone 68 10 compared to control 100 . Epithelial cell secretions induced eosinophil survival from day 1 to 4 this effect being inhibited by mometasone. At day 4 the combination of mometasone 10-11M and desloratadine 10-5M provoked an increased inhibition of eosinophil survival induced by cell secretions 27 5 p
