Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Disaturated-phosphatidylcholine and Surfactant protein-B turnover in human acute lung injury and in control patients. | Simonato et al. Respiratory Research 2011 12 36 http content 12 1 36 RESPIRATORY RESEARCH RESEARCH Open Access Disaturated-phosphatidylcholine and Surfactant protein-B turnover in human acute lung injury and in control patients 1 2 3 1 3 3 Manuela Simonato Aldo Baritussio Carlo Ori Luca Vedovelli Sandra Rossi Lorenza Dalla Massara Sabina Rizzi3 Virgilio P Carnielli4 and Paola E Cogo1 Abstract Background Patients with Adult Respiratory Distress Syndrome ARDS and Acute Lung Injury ALI have low concentrations of disaturated-phosphatidylcholine and surfactant protein-B in bronchoalveolar lavage fluid. No information is available on their turnover. Objectives To analyze disaturated-phosphatidylcholine and surfactant protein-B turnover in patients with ARDS ALI and in human adults with normal lungs controls . Methods 2H2O as precursor of disaturated-phosphatidylcholine-palmitate and 113C-Leucine as precursor of surfactant protein-B were administered intravenously to 12 patients with ARDS ALI and to 8 controls. Disaturated-phosphatidylcholine and surfactant protein-B were isolated from serial tracheal aspirates and their fractional synthetic rate was derived from the 2H and 13C enrichment curves obtained by gas chromatography mass spectrometry. Disaturated-phosphatidylcholine surfactant protein-B and protein concentrations in tracheal aspirates were also measured. Results 1 Surfactant protein-B turned over at faster rate than disaturated-phosphatidylcholine both in ARDS ALI patients and in controls. 2 In patients with ARDS ALI the fractional synthesis rate of disaturated-phosphatidylcholine was times higher than in controls p while the fractional synthesis rate of surfactant protein-B was not different. 3 In ARDS ALI patients the concentrations of disaturated-phosphatidylcholine and surfactant protein-B in tracheal aspirates were markedly and significantly reduced 17 and 40 of the control values respectively . Conclusions 1 .
