Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:" Adrenomedullin in inflammatory process associated with experimental pulmonary fibrosis. | Di Paola et al. Respiratory Research 2011 12 41 http content 12 1 41 RESPIRATORY RESEARCH RESEARCH Open Access Adrenomedullin in inflammatory process associated with experimental pulmonary fibrosis 1 3 2 113 Rosanna Di Paola Elena Talero Maria Galuppo Emanuela Mazzon Placido Bramanti Virginia Motilva and Salvatore Cuzzocrea1 2 Abstract Background Adrenomedullin AM a 52-amino acid ringed-structure peptide with C-terminal amidation was originally isolated from human pheochromocytoma. AM are widely distributed in various tissues and acts as a local vasoactive hormone in various conditions. Methods In the present study we investigated the efficacy of AM on the animal model of bleomycin BLM -induced lung injury. Mice were subjected to intratracheal administration of BLM and were assigned to receive AM daily by an intraperitoneal injection of 200 ngr kg. Results and Discussion Myeloperoxidase activity lung histology immunohistochemical analyses for cytokines and adhesion molecules expression inducible nitric oxide synthase iNOS nitrotyrosine and poly ADP-ribose polymerase PARP were performed one week after fibrosis induction. Lung histology and transforming growth factor beta TGF-b were performed 14 and 21 days after treatments. After bleomycin administration AM-treated mice exhibited a reduced degree of lung damage and inflammation compared with BLM-treated mice as shown by the reduction of 1 myeloperoxidase activity MPO 2 cytokines and adhesion molecules expression 3 nitric oxide synthase expression 4 the nitration of tyrosine residues 5 poly ADP-ribose PAR formation a product of the nuclear enzyme poly ADP-ribose polymerase PARP 6 transforming growth factor beta TGF-b 7 and the degree of lung injury. Conclusions Our results indicate that AM administration is able to prevent bleomycin induced lung injury through the down regulation of proinflammatory factors. Background Idiopathic pulmonary fibrosis IPF is one of the most common forms of .
