Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:" In vivo imaging of the airway wall in asthma: fibered confocal fluorescence microscopy in relation to histology and lung function. | Yick et al. Respiratory Research 2011 12 85 http content 12 1 85 RESPIRATORY RESEARCH RESEARCH Open Access In vivo imaging of the airway wall in asthma fibered confocal fluorescence microscopy in relation to histology and lung function 1 1 1 1 Ching Yong Yick Jan H von der Thusen Elisabeth H Bel Peter J Sterk and Peter W Kunst Abstract Background Airway remodelling is a feature of asthma including fragmentation of elastic fibres observed in the superficial elastin network of the airway wall. Fibered confocal fluorescence microscopy FCFM is a new and non-invasive imaging technique performed during bronchoscopy that may visualize elastic fibres as shown by in vitro spectral analysis of elastin powder. We hypothesized that FCFM images capture in vivo elastic fibre patterns within the airway wall and that such patterns correspond with airway histology. We aimed to establish the concordance between the bronchial elastic fibre pattern in histology and FCFM. Second we examined whether elastic fibre patterns in histology and FCFM were different between asthmatic subjects and healthy controls. Finally the association between these patterns and lung function parameters was investigated. Methods In a cross-sectional study comprising 16 subjects 8 atopic asthmatic patients with controlled disease and 8 healthy controls spirometry and bronchoscopy were performed with recording of FCFM images followed by endobronchial biopsy at the airway main carina. Elastic fibre patterns in histological sections and FCFM images were scored semi-quantitatively. Agreement between histology and FCFM was analysed using linearly weighted kappa Kw. Results The patterns observed in histological sections and FCFM images could be divided into 3 distinct groups. There was good agreement between elastic fibre patterns in histology and FCFM patterns kw . The semi-quantitative pattern scores were not different between asthmatic patients and controls. Notably there was a
