Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"Abnormal spatial diffusion of Ca2+ in F508del-CFTR airway epithelial cells. | Respiratory Research BioMed Central Research Abnormal spatial diffusion of Ca2 in F508del-CFTR airway epithelial cells Fabrice Antigny Caroline Norez Anne Cantereau Frédéric Becq and Clarisse Vandebrouck Open Access Address Institut de Physiologie et Biologie Cellulaires Université de Poitiers CNRS 86022 Poitiers France Email Fabrice Antigny - Caroline Norez - Anne Cantereau - Frédéric Becq - Clarisse Vandebrouck - Corresponding author Published 30 October 2008 Received I April 2008 Respiratory Research 2008 9 70 doi 1465-9921 -9-70 Accepted 30 October 2008 This article is available from http content 9 1 70 2008 Antigny et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background In airway epithelial cells calcium mobilization can be elicited by selective autocrine and or paracrine activation of apical or basolateral membrane heterotrimeric G protein-coupled receptors linked to phospholipase C PLC stimulation which generates inositol 1 4 5-trisphosphate IP3 and 1 2-diacylglycerol DAG and induces Ca2 release from endoplasmic reticulum ER stores. Methods In the present study we monitored the cytosolic Ca2 transients using the UV light photolysis technique to uncage caged Ca2 or caged IP3 into the cytosol of loaded airway epithelial cells of cystic fibrosis CF and non-CF origin. We compared in these cells the types of Ca2 receptors present in the ER and measured their Ca2 dependent activity before and after correction of F508del-CFTR abnormal trafficking either by low temperature or by the pharmacological corrector
