Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"PKA and Epac cooperate to augment bradykinin-induced interleukin-8 release from human airway smooth muscle cells. | Respiratory Research BioMed Central Open Access PKA and Epac cooperate to augment bradykinin-induced interleukin-8 release from human airway smooth muscle cells Sara S Roscioni 1 Loes EM Kistemaker1 Mark H Menzen1 Carolina RS Elzinga1 Reinoud Gosens1 Andrew J Halayko2 Herman Meurs1 and Martina Schmidt1 Address Department of Molecular Pharmacology University of Groningen Groningen The Netherlands and - Departments of Physiology and Internal Medicine University of Manitoba Winnipeg Manitoba Canada Email Sara S Roscioni - Loes EM Kistemaker - Mark H Menzen - Carolina RS Elzinga - Reinoud Gosens - Andrew J Halayko - Ahalayk@ Herman Meurs - Martina Schmidt - Corresponding author Published 29 September 2009 Received 16 May 2009 Respiratory Research 2009 10 88 doi l465-992l-l0-88 Accepted 29 September 2009 This article is available from http content l0 l 88 2009 Roscioni et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Airway smooth muscle contributes to the pathogenesis of pulmonary diseases by secreting inflammatory mediators such as interleukin-8 IL-8 . IL-8 production is in part regulated via activation of Gq-and Gs-coupled receptors. Here we study the role of the cyclic AMP cAMP effectors protein kinase A PKA and exchange proteins directly activated by cAMP Epacl and Epac2 in the bradykinin-induced IL-8 release from a human airway smooth muscle cell line and the underlying molecular mechanisms of this response. Methods IL-8 release was assessed via ELISA under basal condition and after stimulation with bradykinin .
