Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Gene expression profiling in circulating endothelial cells from systemic sclerosis patients shows an altered control of apoptosis and angiogenesis that is modified by iloprost infusion. | Tinazzi et al. Arthritis Research Therapy 2010 12 R131 http content 12 4 R131 RESEARCH ARTICLE Open Access Gene expression profiling in circulating endothelial cells from systemic sclerosis patients shows an altered control of apoptosis and angiogenesis that is modified by iloprost infusion Rlir-i Tin7t 7-7i 1 t l l r 7Í2 F lr tlI inri2 A ntrnrúrn D ir r Q f f i2 3A f ii rtif iCil 1 Ri irti4 D innQrri Rori1 K lorio Toroc Ỉ7ì lont 5 Elisa Ilnazzl Malzla Dolclno Antonlo Puccettl Antonella Rlgo Ruggero Dell IVIdlld leresa valentl Roberto Corrocher1 Claudlo Lunardl1 Abstract Introduction Círculdtíng endothellal cells are lncreased ln patlents affected by systemlc sclerosls SSc and theír number strongly correlates wlth vascular damage. The effects of lloprost ln systemlc sclerosls are only partially known. We aimed at studying the gene expresslon profile of clrculatlng endothellal cells and the effects of lloprost lnfuslon and gene expresslon ln patlents wlth systemlc sclerosls. Methods We enrolled 50 patlents affected by systemlc sclerosls 37 patlents wlthout and 13 patlents wlth dlgltal ulcers. Blood samples were collected from all patlents before and 72 hours after elther a slngle day or five days elght hours lloprost lnfuslon. Blood samples were also collected from 50 sex- and age-matched healthy controls. Clrculatlng endothellal cells and endothellal progenltors cells were detected ln the peripheral blood of patlents wlth systemlc sclerosls by flow cytometry wlth a four-colour panel of antlbodles. Statlstlcal analysls was performed wlth the SPSS 16 statistical endothellal cells were then lsolated from peripheral blood by lmmunomagnetlc CD45 negative selection for the gene array study. Results The number of both clrculatlng endothellal cells and progenltors was slgnlflcantly hlgher ln patients affected by systemlc sclerosls than ln controls and among patlents ln those wlth dlgltal ulcers than ln patlents wlthout them. .
