Báo cáo y học: "Abnormalities in circulating plasmacytoid dendritic cells in patients with systemic lupus erythematosus"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài:Abnormalities in circulating plasmacytoid dendritic cells in patients with systemic lupus erythematosus. | Jin et al. Arthritis Research Therapy 2010 12 R137 http content 12 4 R137 RESEARCH ARTICLE Open Access Abnormalities in circulating plasmacytoid dendritic cells in patients with systemic lupus erythematosus Ou Jin 1 2 Sushma Kavikondala1 Mo-Yin Mok1 Lingyun Sun3 Jieruo Gu2 Rong Fu1 Albert Chan1 Joseph Yeung1 Yingjie Nie1 and Chak-Sing Lau 1 Abstract Introduction Dendritic cells DCs are capable of inducing immunity or tolerance. Previous studies have suggested plasmacytoid DCs pDCs are pathogenic in systemic lupus erythematosus SLE . However the functional characteristics of directly isolated peripheral circulating blood pDCs in SLE have not been evaluated previously. Methods Peripheral blood pDCs from 62 healthy subjects and 58 SLE patients were treated with apoptotic cells derived from polymorphonuclear cells PMNs . Antigen loaded or unloaded pDCs were then co-cultured with autologous or allogenous T cells. Changes in T cell proliferation cell surface CD25 expression intracellular Foxp3 expression and cytokine production were evaluated. pDCs that had captured apoptotic PMNs pDCs apoPMNs were also studied for their cytokine production interferon IFN -alpha interleukin IL -6 IL-10 IL-18 and toll like receptor TLR expression. Results Circulating pDCs from SLE patients had an increased ability to stimulate T cells when compared with control pDCs. Using allogenous T cells as responder cells SLE pDCs induced T cell proliferation even in the absence of apoptotic PMNs. In addition healthy pDCs apoPMNs induced suppressive T regulatory cell features with increased Foxp3 expression in CD4 CD25 cells while SLE pDCs apoPMNs did not. There were differences in the cytokine profile of pDCs that had captured apoptotic PMNs between healthy subjects and patients with SLE. Healthy pDCs apoPMNs showed decreased production of IL-6 but no significant changes in IL-10 and IL-18. These pDCs apoPMNs also showed increased mRNA transcription of TLR9. On the other hand

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