Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài:Pharmacoproteomic study of the effects of chondroitin and glucosamine sulfate on human articular chondrocytes. | Calamia et al. Arthritis Research Therapy 2010 12 R138 http content 12 4 R138 RESEARCH ARTICLE Open Access Phaịrmacoproteomic study of the effects of chondroitin and glucosamine sulfate on human articular chondrocytes Valentina Calamia 1 Cristina Ruiz-Romero1 Beatriz Rocha1 Patricia Fernandez-Puente1 Jesus Mateos1 Eulàlia Montell2 Josep Vergés2 and Francisco J Blanco 1 Abstract Introduction Chondroitin sulfate CS and glucosamine sulfate GS are symptomatic slow-acting drugs for osteoarthritis OA widely used in clinic. Despite their widespread use knowledge of the specific molecular mechanisms of their action is limited. The aim of this work is to explore the utility of a pharmacoproteomic approach for the identification of specific molecules involved in the pharmacological effect of GS and CS. Methods Chondrocytes obtained from three healthy donors were treated with GS 10 mM and or CS 200 pg mL and then stimulated with interleukin-1 p IL-1 P 10 ng mL. Whole cell proteins were isolated 24 hours later and resolved by two-dimensional electrophoresis. The gels were stained with SYPRORuby. Modulated proteins were identified by matrix-assisted laser desorption ionization time-of-flight MALDI-TOF TOF mass spectrometry. Real-time PCR and Western blot analyses were performed to validate our results. Results A total of 31 different proteins were altered by GS or and CS treatment when compared to control. Regarding their predicted biological function 35 of the proteins modulated by GS are involved in signal transduction pathways 15 in redox and stress response and 25 in protein synthesis and folding processes. Interestingly CS affects mainly energy production 31 and metabolic pathways 13 decreasing the expression levels of ten proteins. The chaperone GRP78 was found to be remarkably increased by GS alone and in combination with CS a fact that unveils a putative mechanism for the reported anti-inflammatory effect of GS in OA. On the other hand the .
