Tuyển tập các báo cáo nghiên cứu về bệnh thú y được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành thú y đề tài: In Vitro Evaluation of Frozen-Thawed Stallion Semen: A Review. | Acta vet. scand. 2001 42 199-217. In Vitro Evaluation of Frozen-Thawed Stallion Semen A Review By T. Katila Department of Clinical Veterinary Sciences University of Helsinki Finland. Katila T. In vitro evaluation of frozen-thawed stallion semen. A review. Acta vet. scand. 2000 42 201-217. - The article reviews methods used for in vitro evaluation of sperm with particular emphasis on frozen-thawed stallion sperm. The techniques limitations of the methods and correlations with fertility results are discussed. Very few studies have tried to find correlation between fertility of frozen stallion semen and laboratory tests. It is difficult and expensive to inseminate an adequate number of mares to achieve statistically significant differences. Significant but low correlations have been demonstrated between the foaling rate and subjective motility of sperm incubated for 2 h and 4 h at 37 C and hypoosmotic swelling test after 0 and 3 h of incubation. Significant correlations have been reported between the pregnancy rate and viability of pro-pidium iodide-stained sperm assessed by flow cytometry as well as for glass wool and Sephadex filtration tests. No correlations have been detected between fertility and motility immediately after thawing. In spite of that motility estimation by light microscope is the most commonly used method to evaluate frozen-thawed stallion sperm. Computer assisted automatic sperm analyzers have replaced light microscopy in research projects but so far nobody has been able to demonstrate a correlation between fertility of frozen stallion semen and any of the motility parameters obtained by these instruments. horse sperm cryopreservation semen quality motility membrane integrity. Introduction There is considerable variation between individual stallions in how well their semen retains its fertilizing capacity after freezing and thawing. It has been estimated that only 20 of fertile stallions produce sperm that survive well the freezing and thawing .
