Tuyển tập các báo cáo nghiên cứu về bệnh thú y được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành thú y đề tài: Plasma Calcium, Inorganic Phosphate and Magnesium During Hypocalcaemia Induced by a Standardized EDTA Infusion in Cows. | Acta vet. scand. 2001 42 251-260. Plasma Calcium Inorganic Phosphate and Magnesium During Hypocalcaemia Induced by a Standardized EDTA Infusion in Cows By . Mellau . Jorgensen and . Enemark Cattle Production Medicine Research Group Clinical Department Large Animal Medicine The Royal Veterinary and Agricultural University Frederiksberg Denmark. Mellau LSB Jorgensen RJ Enemark JMD Plasma calcium inorganic phosphate and magnesium during hypocalcaemia induced by a standardized EDTA infusion in cows. Acta vet. scand. 2001 42 251-260. - The intravenous Na2EDTA infusion technique allows effective specific chelation of circulating Ca2 leading to a progressive hypocalcaemia. Methods previously used were not described in detail and results obtained by monitoring total and free ionic calcium were not comparable due to differences in sampling and analysis. This paper describes a standardized EDTA infusion technique that allowed comparison of the response of calcium phosphorus and magnesium between 2 groups of experimental cows. The concentration of the Na2EDTA solution was mol l and the flow rate was standardized at ml kg per hour. Involuntary recumbency occurred when ionised calcium dropped to - mmol l due to chelation. An initial fast drop of ionized calcium was observed during the first 20 min of infusion followed by a fluctuation leading to a further drop until recumbency. Pre-infusion Ca2 between tests does not correlate with the amount of EDTA required to induce involuntary recumbence. Total calcium concentration measured by atomic absorption remained almost constant during the first 100 min of infusion but declined gradually when the infusion was prolonged. The concentration of inorganic phosphate declined gradually in a fluctuating manner until recumbency. Magnesium concentration remained constant during infusion. Such electrolyte responses during infusion were comparable to those in spontaneous milk fever. The standardized infusion .
