Báo cáo khoa học: " An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus)"

Tuyển tập các báo cáo nghiên cứu về bệnh thú y được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành thú y đề tài: An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus). | Acta vet. scand. 2002 43 211-220. An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes Alopex lagopus By J. Ằkerstedt National Veterinary Institute Oslo Norway Ảkerstedt J An indirect ELISA for detection of Encephalitozoon cuniculi infection in farmed blue foxes Alipex lagopus . Acta vet. scand. 2002 43 211-220. - Infection with the intracellular microsporidium Encephalitozoon cuniculi can cause serious disease encephalitozoonosis in the blue fox Alopex lagopus . The disease diagnosis is based on clinical signs and pathological findings and detection of E. cuniculi or circulating antibodies directed against the parasite. Indirect immunofluorescence IFAT and carbon immunoassay CIA are the most commonly used serological methods for diagnosis in this species. In the present study an indirect ELISA enzyme linked immunosorbent assay was established and evaluated against IFAT by testing of 205 field samples from blue foxes. There was high agreement between the results of the ELISA and CIA k and the ELISA and IFAT k . There was no significant statistical difference between the tests p . It was concluded that the ELISA could be used to identify seropositive farmed blue foxes. The advantage of the ELISA lies in the potential of screening large numbers of animals with the goal of eradicating E. cuniculi infection in the farms. microsporidia encephalitozoonosis ELISA carbon immunoassay indirect immunofluorescence. Introduction The intracellular microsporidium Encephalito-zoon cuniculi is an obligate parasite that infects a wide range of vertebrate animals Canning Lom 1986 . In some hosts such as rabbit and carnivores infection can cause disease. The development of clinical disease in different species is highly influenced by natural innate species susceptibility and by the immune status of the individual. For example severe disease can occur in young dogs or in the blue fox Alopex lagopus whereas the farmed silver fox .

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