Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Alterations in cytoskeletal and immune function-related proteome profiles in whole rat lung following intratracheal instillation of heparin. | Respiratory Research BioMed Central Research Open Access Alterations in cytoskeletal and immune function-related proteome profiles in whole rat lung following intratracheal instillation of heparin Amir A Gabr1 Mathew Reed2 Donna R Newman1 Jan Pohl2 Jody Khosla1 and Philip L Sannes 1 Address Department of Molecular Biomedical Sciences Center for Comparative Molecular Medicine and Translational Research College of Veterinary Medicine North Carolina State University Raleigh NC USA and 2Microchemical and Proteomics Facility Winship Cancer Institute School of Medicine Emory University Atlanta GA USA Email Amir A Gabr - amirgabr@ Mathew Reed - mreed01@ Donna R Newman - donna_newman@ Jan Pohl - jpohl@ Jody Khosla - jody_khosla@ Philip L Sannes - philip_sannes@ Corresponding author Published 8 May 2007 Received 12 January 2007 Respiratory Research 2007 8 36 doi l465-992l-8-36 Accepted 8 May 2007 This article is available from http content 8 l 36 2007 Gabr et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Heparin has been shown to modify fundamental biologic processes ranging from blood coagulation and cell proliferation to fibrogenesis and asthma. The goal of this study was to identify specific or broad biologic responses of the rat lung to intratracheal instillation of heparin by targeted proteomic analysis. Methods Rats were given either aerosolized 500 pg heparin in 250 pl saline or saline alone. Lungs were harvested at 0 24 or 96 hours post-treatment and isolated proteins analyzed by twodimensional gel electrophoresis. Proteins which increased and decreased significantly in treated groups above controls were then selected
