Báo cáo khoa học: " IS900 Restriction Fragment Length Polymorphism (RFLP) Analysis of Mycobacterium avium subsp. paratuberculosis Isolates from Goats and Cattle in Norway"

Tuyển tập các báo cáo nghiên cứu về bệnh thú y được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành thú y đề tài: IS900 Restriction Fragment Length Polymorphism (RFLP) Analysis of Mycobacterium avium subsp. paratuberculosis Isolates from Goats and Cattle in Norway. | Acta vet. scand. 2005 46 13-18. IS900 Restriction Fragment Length Polymorphism RFLP Analysis of Mycobacterium avium subsp. paratuberculosis Isolates from Goats and Cattle in Norway By B. Dj0nne1 1. Pavlik2 P Svastova2 M. Bartos2 and G. Holstad1 department of bacteriology National Veterinary Institute Post Box 8156 Dep. N-0033 Oslo Norway 2Veteri-nary Research Institute Hudcova 70 Brno 621 32 Czech republic Djonne B Pavlik I Svastova P Bartos M and Holstad G IS900 restriction fragment length polymorphism RFLP analysis of Mycobacterium avium subsp. paratuberculosis isolates from goats and cattle in Norway. Acta vet. scand. 2005 46 13-18. - In Norway paratuberculosis has been frequently diagnosed in goats while cattle have been almost free of the infection. This difference in prevalence between goats and cattle has led to speculations about the existence of a Mycobacterium avium subsp. paratuberculosis M. a. paratuberculosis isolate that is non-pathogenic for cattle. There is little information available on genotypic variation of M. a. paratuberculosis isolated from animals in Norway. In the present study genotypic information on 51 isolates from goats and four isolates from cattle in Norway was obtained by use of IS900 restriction fragment length polymorphism RFLP analysis. All isolates from cattle and 84 of the isolates from goats had the same RFLP pattern B-C1 . Five RFLP patterns not previously detected were found. No genotypic variation that could explain a difference in host origin was found between the isolates from cattle and the majority of the Norwegian goat isolates. This lack of difference indicates that the most common M. a. paratuberculosis isolates in Norway may infect both cattle and goats. Mycobacterium avium subsp. paratuberculosis Strain characterisation RFLP fingerprint Goat Cattle. Introduction Paratuberculosis is a chronic intestinal inflammation in ruminants caused by Mycobacterium avium subsp. paratuberculosis M. a. paratuberculosis . The .

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