Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: A GA microsatellite in the Fli1 promoter modulates gene expression and is associated with systemic lupus erythematosus patients without nephritis. | Morris et al. Arthritis Research Therapy 2010 12 R212 http content 12 6 R212 RESEARCH ARTICLE Open Access A GA microsatellite in the Flil promoter modulates gene expression and is associated with systemic lupus erythematosus patients without nephritis 11 2 111 Erin E Morris May Y Amria Emily Kistner-Griffin John L Svenson Diane L Kamen Gary S Gilkeson Tamara K Nowling1 Abstract Introduction The transcription factor Fli1 is implicated in the pathogenesis of systemic lupus erythematosus SLE . Recently a GAn polymorphic microsatellite was characterized in the mouse Flil promoter that modulates promoter activity and is truncated in two lupus mouse models compared to non-autoimmune prone mice. In this work we characterize a homologous GAn microsatellite in the human Flil promoter. The purpose of this study is to determine the effect of the microsatellite length on Flil promoter activity in vitro and to determine if the length of the GAn microsatellite is associated with SLE and or specific disease characteristics. Methods Constructs with variable lengths of the GAn microsatellite in the Flil promoter were generated and analyzed in promoter reporter P R assays in a human T cell line. Using three SLE patient cohorts and matched controls microsatellite length was measured and association with the presence of disease and the occurrence of specific disease manifestations was assessed. Results P R assays demonstrated that the presence of a shorter microsatellite resulted in higher Flil promoter activity. A significant association was observed in the lupus cohort SLE in Gullah Health SLEIGH between the GA26 base pair allele and absence of nephritis. Conclusions This study demonstrates that a GAn microsatellite in the human Fli1 promoter is highly polymorphic. The length of the microsatellite is inversely correlated to Flil promoter activity in a human T cell line. Although no association between microsatellite length and lupus was observed an association
