Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Salivary glands of primary Sjögren’s syndrome patients express factors vital for plasma cell survival. | Szyszko et al. Arthritis Research Therapy 2011 13 R2 http content 13 1 R2 RESEARCH ARTICLE Open Access Salivary glands of primary Sjogren s syndrome patients express factors vital for plasma cell survival Ewa A Szyszko1 2 Karl A Brokstad1 Gunnvor 0ijordsbakken2 Malin V Jonsson3 Roland Jonsson1 4 Kathrine Skarstein2 Abstract Introduction The presence of circulating Ro SSA and La SSB autoantibodies has become an important marker in the classification criteria for primary Sjogren s syndrome pSS . Plasma cells producing these autoantibodies are mainly high affinity plasma cells originating from germinal centre reactions. When exposed to the right microenvironment these autoimmune plasma cells become long-lived and resistant to immunosuppressive treatment. Since autoimmune plasma cells have been detected in the salivary glands of SS patients we wanted to investigate if the glandular microenvironment is suitable for plasma cell survival and if glandular residing plasma cells are the long-lived plasma cell subset. Methods Single double and triple immunohistochemistry as well as immunofluorescence staining was performed on minor salivary gland tissue retrieved from pSS chronically inflamed and normal subjects. Results We detected significant numbers of CD138 non-proliferating Bcl-2 expressing plasma cells in the salivary glands of pSS patients with high focus score FS . Furthermore we demonstrated that CXCL12 and interleukin IL -6 survival factors were highly expressed in pSS salivary gland epithelium and by focal mononuclear infiltrating cells. Notably adipocytes when present in the salivary gland tissue were an important source of CXCL12. We clearly demonstrate that plasma cells are localised in close proximity to CXCL12 and IL-6 expressing cells and thus that the environment of salivary glands with high FS provide factors vital for plasma cell survival. Conclusions Plasma cells residing in the salivary glands of pSS patients with high FS showed .
