Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Relative percentage and zonal distribution of mesenchymal progenitor cells in human osteoarthritic and normal cartilage. | Pretzel et al. Arthritis Research Therapy 2011 13 R64 http content 13 2 R64 RESEARCH ARTICLE Open Access Relative percentage and zonal distribution of mesenchymal progenitor cells in human osteoarthritic and normal cartilage 1133 4 David Pretzel 1 Stefanie Linss Steffen Rochler Michaela Endres Christian Kaps Saifeddin Alsalameh and Raimund W Kinne1 Abstract Introduction Mesenchymal stem cells MSC are highly attractive for use in cartilage regeneration. To date MSC are usually recruited from subchondral bone marrow using microfracture. Recent data suggest that isolated cells from adult human articular cartilage which express the combination of the cell-surface markers CD105 and CD166 are multi-potent mesenchymal progenitor cells MPC with characteristics similar to MSC. MPC within the cartilage matrix the target of tissue regeneration may provide the basis for in situ regeneration of focal cartilage defects. However there is only limited information concerning the presence abundance of CD105 CD166 MPC in human articular cartilage. The present study therefore assessed the relative percentage and particularly the zonal distribution of cartilage MPC using the markers CD105 CD166. Methods Specimens of human osteoarthritic OA n 11 and normal n 3 cartilage were used for either cell isolation or immunohistochemistry. Due to low numbers isolated cells were expanded for 2 weeks and then analyzed by flow cytometry FACS or immunofluorescence in chamber slides for the expression of CD105 and CD166. Following immunomagnetic separation of CD166 - OA cells multi-lineage differentiation assays were performed. Also the zonal distribution of CD166 cells within the matrix of OA and normal cartilage was analyzed by immunohistochemistry. Results FACS analysis showed that mean SEM of OA and of normal chondrocytes . were CD105 CD166 and thus carried the established MPC marker combination. Similarly and of CD105 CD166 cells .
