Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: High mobility group box 1 (HMGB1) and anti-HMGB1 antibodies and their relation to disease characteristics in systemic lupus erythematosus. | Abdulahad et al. Arthritis Research Therapy 2011 13 R71 http content 13 3 R71 RESEARCH ARTICLE Open Access High mobility group box 1 HMGB1 and anti-HMGB1 antibodies and their relation to disease characteristics in systemic lupus erythematosus Deena A Abdulahad Johanna Westra Johannes Bijzet Pieter C Limburg Cees GM Kallenberg and Marc Bijl Abstract Introduction High Mobility Group Box 1 HMGB1 is a nuclear non-histone protein. HMGB1 which is secreted by inflammatory cells and passively released from apoptotic and necrotic cells may act as a pro-inflammatory mediator. As apoptotic cells accumulate in systemic lupus erythematosus SLE HMGB1 levels might be increased in SLE. HMGB1 may also serve as an autoantigen leading to the production of anti-HMGB1 antibodies. In this study we determined levels of HMGB1 and anti-HMGB1 in SLE patients in comparison to healthy controls HC and analysed their relation with disease activity. Methods The study population consisted of 70 SLE patients and 35 age- and sex-matched HC. Thirty-three SLE patients had quiescent disease the other 37 patients were selected for having active disease. Nineteen of these had lupus nephritis. HMGB1 levels were measured with both Western blot and ELISA. Anti-HMGB1 levels were measured by ELISA. Clinical and serological parameters were assessed according to routine procedures. Results HMGB1 levels in SLE patients could be measured reliably by Western blotting only and were significantly increased compared to HC. During active disease HMGB1 levels increased in particular in patients with renal involvement. Serum HMGB1 levels correlated with SLEDAI proteinuria and anti-dsDNA levels and showed a negative correlation with complement C3. Anti-HMGB1 levels were significantly increased in SLE patients compared to HC and positively correlated with HMGB1 levels. Conclusions Levels of HMGB1 in the sera of SLE patients in particular in those with active renal disease are increased. Serum HMGB1
