Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Histone deacetylase inhibition alters dendritic cells to assume a tolerogenic phenotype and ameliorates arthritis in SKG mice. | Misaki et al. Arthritis Research Therapy 2011 13 R77 http content 13 3 R77 RESEARCH ARTICLE Open Access Histone deacetylase inhibition alters dendritic cells to assume a tolerogenic phenotype and ameliorates arthritis in SKG mice 1 2 1 1 1 Kenta Misaki Akio Morinobu Jun Saegusa Shimpei Kasagi Masaaki Fujita Yoshiaki Miyamoto Fumichika Matsuki2 and Shunichi Kumagai1 2 Abstract Introduction The purpose of this study was to elucidate the effects of histone deacetylase inhibition on the phenotype and function of dendritic cells and on arthritis in SKG mice. Methods Arthritis was induced in SKG mice by zymosan A injection. Trichostatin A a histone deacetylase inhibitor was administered and its effects on arthritis were evaluated by joint swelling and histological evaluation. Interleukin-17 production in lymph node cells was determined by an enzyme-linked immunosorbent assay ELISA . Foxp3 expression in lymph node cells and the phenotypes of splenic dendritic cells were examined by fluorescence-activated cell sorting FACS . Bone marrow-derived dendritic cells BM-DC were generated with granulocyte macrophage colony-stimulating factor. The effects of trichostatin A on cell surface molecules cytokine production indoleamine 2 3-dioxygenase IDO expression and T cell stimulatory capacity were examined by FACS ELISA quantitative real-time polymerase chain reaction and Western blot and the allo-mixed lymphocyte reaction respectively. Results Trichostatin A when administered before the onset of arthritis prevented SKG mice from getting arthritis. Trichostatin A treatment also showed therapeutic effects on arthritis in SKG mice when it was administered after the onset of arthritis. Trichostatin A treatment reduced Th17 cells and induced regulatory T cells in lymph node and also decreased co-stimulatory molecule expression on splenic dendritic cells in vivo. In vitro trichostatin A markedly suppressed zymosan A-induced interleukin-12 and interleukin-6 .
