Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Differential regulation of neurotrophin expression in human bronchial smooth muscle cells. | Respiratory Research BioMed Central Research Open Access Differential regulation of neurotrophin expression in human bronchial smooth muscle cells Cecilia Kemi1 Johan Grunewald1 Anders Eklund1 and Caroline Olgart Hoglund 1 2 Address department of Medicine Division of Respiratory Medicine Lung Research Laboratory Karolinska Institutet and Karolinska University Hospital Solna 171 76 Stockholm Sweden and department of Physiology and Pharmacology Karolinska Institutet 171 77 Stockholm Sweden Email Cecilia Kemi - Johan Grunewald - Anders Eklund - Caroline Olgart Hoglund - Corresponding author Published 29 January 2006 Respiratory Research 2006 7 18 doi l465-992l-7-l8 Received 22 September 2005 Accepted 29 January 2006 This article is available from http content 7 l l8 2006 Kemi et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Human bronchial smooth muscle cells HBSMC may regulate airway inflammation by secreting cytokines chemokines and growth factors. The neurotrophins including nerve growth factor NGF brain-derived neurotrophic factor BDNF and neurotrophin-3 NT-3 have been shown to be elevated during airway inflammation and evoke airway hyperresponsiveness. We studied if HBSMC may be a source of NGF BDNF and NT-3 and if so how inflammatory cytokines may influence their production. Methods Basal and cytokine IL-1P IFN-Y IL-4 -stimulated neurotrophin expression in HBSMC cultured in vitro was quantified. The mRNA expression was quantified by real-time RT-PCR and the protein secretion into the cell culture medium by ELISA. Results We observed a constitutive NGF BDNF and NT-3 .
