Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Allergens induce enhanced bronchoconstriction and leukotriene production in C5 deficient mice. | Respiratory Research BioMed Central Research Open Access Allergens induce enhanced bronchoconstriction and leukotriene production in C5 deficient mice Laura McKinley1 Jiyoun Kim1 Gerald L Bolgos1 Javed Siddiqui1 and Daniel G Remick 1 2 Address Department of Pathology University of Michigan Ann Arbor MI USA and 2Boston University School of Medicine Department of Pathology 670 Albany Street Room 407 Boston MA 02118 USA Email Laura McKinley - Jiyoun Kim - Jiyoukim@ Gerald L Bolgos - Gbolgos@ Javed Siddiqui - Siddiqui@ Daniel G Remick - remickd@ Corresponding author Published 17 October 2006 Received 03 February 2006 Respiratory Research 2006 7 129 doi 1465-9921-7-129 Accepted 1 5 7 October 2006 This article is available from http content 7 1 129 2006 McKinley et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Previous genetic analysis has shown that a deletion in the complement component 5 gene-coding region renders mice more susceptible to allergen-induced airway hyperresponsiveness AHR due to reduced IL-12 production. We investigated the role of complement in a murine model of asthma-like pulmonary inflammation. Methods In order to evaluate the role of complement B10 mice either sufficient or deficient in C5 were studied. Both groups of mice immunized and challenged with a house dust extract HDE containing high levels of cockroach allergens. Airways hyper-reactivity was determined with wholebody plesthysmography. Bronchoalveolar lavage BAL was performed to determine pulmonary cellular recruitment and measure inflammatory mediators. Lung homogenates were assayed for mediators and plasma levels of IgE determined.
