Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí y học Molecular Biology cung cấp cho các bạn kiến thức về ngành sinh học đề tài: Decrease in Shiga toxin expression using a minimal inhibitory concentration of rifampicin followed by bactericidal gentamicin treatment enhances survival of Escherichia coli O157: H7-infected BALB/c mice. | Rahal et al. Annals of Clinical Microbiology and Antimicrobials 2011 10 34 http content 10 1 34 ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS RESEARCH Open Access Decrease in Shiga toxin expression using a minimal inhibitory concentration of rifampicin followed by bactericidal gentamicin treatment enhances survival of Escherichia coli O157 H7-infected BALB c mice Elias A Rahal Natalie Kazzi Ahmad Sabra Alexander M Abdelnoor and Ghassan M MataP Abstract Background Treatment of Escherichia coli O157 H7 infections with antimicrobial agents is controversial due to an association with potentially fatal sequelae. The production of Shiga toxins is believed to be central to the pathogenesis of this organism. Therefore decreasing the expression of these toxins prior to bacterial eradication may provide a safer course of therapy. Methods The utility of decreasing Shiga toxin gene expression in E. coli O157 H7 with rifampicin prior to bacterial eradication with gentamicin was evaluated in vitro using real-time reverse-transcription polymerase chain reaction. Toxin release from treated bacterial cells was assayed for with reverse passive latex agglutination. The effect of this treatment on the survival of E. coli O157 H7-infected BALB c mice was also monitored. Results Transcription of Shiga toxin-encoding genes was considerably decreased as an effect of treating E. coli O157 H7 in vitro with the minimum inhibitory concentration MIC of rifampicin followed by the minimum bactericidal concentration MBC of gentamicin 99 decrease compared to treatment with gentamicin alone 50-75 decrease . The release of Shiga toxins from E. coli O157 H7 incubated with the MIC of rifampicin followed by addition of the MBC of gentamicin was decreased as well. On the other hand the highest survival rate in BALB c mice infected with E. coli O157 H7 was observed in those treated with the in vivo MIC equivalent dose of rifampicin followed by the in vivo MBC equivalent dose .
