Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Abnormal networks of immune response-related molecules in bone marrow cells from patients with rheumatoid arthritis as revealed by DNA microarray analysis. | Lee et al. Arthritis Research Therapy 2011 13 R89 http content 13 3 R89 RESEARCH ARTICLE Open Access Abnormal networks of immune response-related molecules in bone marrow cells from patients with rheumatoid arthritis as revealed by DNA microarray analysis Hooi-Ming Lee1 Hidehiko Sugino1 Chieko Aoki2 Yasunori Shimaoka3 Ryuji Suzuki4 Kensuke Ochi5 Takahiro Ochi6 and Norihiro Nishimoto1 2 Abstract Introduction Rheumatoid arthritis RA is a systemic autoimmune disease characterized by chronic synovitis that progresses to destruction of cartilage and bone. Bone marrow BM cells have been shown to contribute to this pathogenesis. In this study we compared differentially expressed molecules in BM cells from RA and osteoarthritis OA patients and analyzed abnormal regulatory networks to identify the role of BM cells in RA. Methods Gene expression profiles GEPs in BM-derived mononuclear cells from 9 RA and 10 OA patients were obtained by DNA microarray. Up- and down-regulated genes were identified by comparing the GEPs from the two patient groups. Bioinformatics was performed by Expression Analysis Systemic Explorer EASE based on gene ontology followed by network pathway analysis with Ingenuity Pathways Analysis IPA . Results The BM mononuclear cells showed 764 up-regulated and 1 910 down-regulated genes in RA patients relative to the OA group. EASE revealed that the gene category response to external stimulus which included the gene category immune response was overrepresented by the up-regulated genes. So too were the gene categories signal transduction and phosphate metabolism. Down-regulated genes were dominantly classified in three gene categories cell proliferation which included mitotic cell cycle DNA replication and chromosome cycle and DNA metabolism. Most genes in these categories overlapped with each other. IPA analysis showed that the up-regulated genes in immune response were highly relevant to the antigen presentation pathway and to
