Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: spondyloarthritis suggests that the innate immune pathway might be of greater relevance than the Th17-mediated adaptive immune response. | Appel et al. Arthritis Research Therapy 2011 13 R95 http content 13 3 R95 RESEARCH ARTICLE Open Access Analysis of IL-17 cells in facet joints of patients with spondyloarthritis suggests that the innate immune pathway might be of greater relevance than the Th17-mediated adaptive immune response 1 1 1 3 4 2 Heiner Appel René Maier Peihua Wu Rebecca Scheer Axel Hempfing Ralph Kayser Andreas Thiel Andreas Radbruch2 Christoph Loddenkemper5 and Joachim Sieper1 2 Abstract Introduction In this study we analysed the number of IL-17 cells in facet joints in the peripheral blood PB and synovial fluid SF of spondyloarthritis SpA patients and compared these results with those of patients with other rheumatic diseases and controls. Methods Immunohistochemical analysis of IL-17 cells was performed in facet joints of 33 ankylosing spondylitis AS patients and compared with data from 20 osteoarthritis OA patients. The frequency of IL-17 CD4 T cells in PB and SF of SpA patients PB n 30 SF n 11 rheumatoid arthritis RA patients PB n 14 SF n 7 OA patients PB n 10 and healthy controls PB n 12 was analysed after stimulation with Staphylococcus aureus Enterotoxin B and phorbol 12-myristate 13-acetate ionomycin and quantified by flow cytometry. Results In AS facet joints the frequency of IL-17-secreting cells was significantly higher than in samples obtained from OA patients P with a slight predominance of IL-17 cells among the mononuclear cells compared to cells with polysegmental nuclei. Immunofluorescence microscopy revealed that the majority of IL-17 cells were myeloperoxidase-positive high-power field HPF and CD15 neutrophils HPF while CD3 T cells HPF and AA-1 mast cells HPF were less often IL-17positive. The frequency of IL-17 CD4 T cells in the PB and SF of SpA patients did not differ significantly compared to RA patients OA patients or healthy controls. Conclusions Our data suggest an important .
