Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Tiludronate treatment improves structural changes and symptoms of osteoarthritis in the canine anterior cruciate ligament model. | Moreau et al. Arthritis Research Therapy 2011 13 R98 http content 13 3 R98 RESEARCH ARTICLE Open Access Tiludronate treatment improves structural changes and symptoms of osteoarthritis in the canine anterior cruciate ligament model 1 2 2 2 Maxim Moreau 1 Pascale Rialland Jean-Pierre Pelletier Johanne Martel-Pelletier Daniel Lajeunesse 2 2 1 1 1 1 Christelle Boileau Judith Caron Diane Frank Bertrand Lussier Jerome RE del Castillo Guy Beauchamp Dominique Gauvin1 2 Thierry Bertaim3 Dominique Thibaud4 and Eric Troncy1 2 Abstract Introduction The aim of this prospective randomized controlled double-blind study was to evaluate the effects of tiludronate TLN a bisphosphonate on structural biochemical and molecular changes and function in an experimental dog model of osteoarthritis OA . Methods Baseline values were established the week preceding surgical transection of the right cranial anterior cruciate ligament with eight dogs serving as OA placebo controls and eight others receiving four TLN injections 2 mg kg subcutaneously at two-week intervals starting the day of surgery for eight weeks. At baseline Week 4 and Week 8 the functional outcome was evaluated using kinetic gait analysis telemetered locomotor actimetry and video-automated behaviour capture. Pain impairment was assessed using a composite numerical rating scale NRS a visual analog scale and electrodermal activity EDA . At necropsy Week 8 macroscopic and histomorphological analyses of synovium cartilage and subchondral bone of the femoral condyles and tibial plateaus were assessed. Immunohistochemistry of cartilage matrix metalloproteinase MMP -1 MMP-13 and a disintegrin and metalloproteinase domain with thrombospondin motifs ADAMTS5 and subchondral bone cathepsin K was performed. Synovial fluid was analyzed for inflammatory PGE2 and nitrite nitrate levels biomarkers. Statistical analyses mixed and generalized linear models were performed with an a-threshold of . Results A better
