Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: CD23+/CD21hi B-cell translocation and ipsilateral lymph node collapse is associated with asymmetric arthritic flare in TNF-Tg mice. | Li et al. Arthritis Research Therapy 2011 13 R138 http content 13 4 R138 RESEARCH ARTICLE Open Access CD23 CD21hi B-cell translocation and ipsilateral lymph node collapse is associated with asymmetric arthritic flare in TNF-Tg mice Jie Li1 2 Quan Zhou3 Ronald W Wood4 5 Igor Kuzin6 Andrea Bottaro2 6 Christopher T Ritchlin1 6 Lianping Xing1 and Edward M Schwarz1 2 5 Abstract Introduction Rheumatoid arthritis RA is a chronic autoimmune disease with episodic flares in affected joints. However how arthritic flare occurs only in select joints during a systemic autoimmune disease remains an enigma. To better understand these observations we developed longitudinal imaging outcomes of synovitis and lymphatic flow in mouse models of RA and identified that asymmetric knee flare is associated with ipsilateral popliteal lymph node PLN collapse and the translocation of CD23 CD21hi B-cells B-in into the paracortical sinus space of the node. In order to understand the relationship between this B-in translocation and lymph drainage from flaring joints we tested the hypothesis that asymmetric tumor necrosis factor TNF -induced knee arthritis is associated with ipsilateral PLN and iliac lymph node ILN collapse B-in translocation and decreased afferent lymphatic flow. Methods TNF transgenic Tg mice with asymmetric knee arthritis were identified by contrast-enhanced CE magnetic resonance imaging MRI and PLN were phenotyped as expanding or collapsed using LNcap threshold 30 Arbitrary Unit AU . Inflammatory-erosive arthritis was confirmed by histology. Afferent lymphatic flow to PLN and ILN was quantified by near infrared imaging of injected indocyanine green NIR-ICG . The B-in population in PLN and ILN was assessed by immunohistochemistry IHC and flow cytometry. Linear regression analyses of ipsilateral knee synovial volume and afferent lymphatic flow to PLN and ILN were performed. Results Afferent lymph flow to collapsed nodes was significantly lower P than
