Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: What have transgenic and knockout animals taught us about respiratory disease. | http content 1 2 082 Review What have transgenic and knockout animals taught us about respiratory disease Yanira Riffo Vasquez and Domenico Spina The Sackler Institute of Pulmonary Pharmacology King s College London London UK Received 26 June 2000 Accepted 18 July 2000 Published 3 August 2000 Respir Res 2000 1 82-86 Current Science Ltd Print ISSN 1465-9921 Online ISSN 1465-993X Abstract Over the past decade there has been a significant shift to the use of murine models for investigations into the molecular basis of respiratory diseases including asthma and chronic obstructive pulmonary disease. These models offer the exciting prospect of dissecting the complex interaction between cytokines chemokines and growth related peptides in disease pathogenesis. Furthermore the receptors and the intracellular signalling pathways that are subsequently activated are amenable for study because of the availability of monoclonal antibodies and techniques for targeted gene disruption and gene incorporation for individual mediators receptors and proteins. However it is clear that extrapolation from these models to the human condition is not straightforward as reflected by some recent clinical disappointments. This is not necessarily a problem with the use of mice itself but results from our continued ignorance of the disease process and how to improve the modelling of complex interactions between different inflammatory mediators that underlie clinical pathology. This review highlights some of the strengths and weaknesses of murine models of respiratory disease. Keywords asthma chemokines cytokines inflammation murine Introduction The incidence of respiratory diseases such as asthma and chronic obstructive pulmonary disease COPD continue to increase despite the availability of current methods of treatment and there is therefore a need to improve our understanding of the pathophysiology of these diseases to permit the development of novel therapeutic agents.
