Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Upregulation of nitric oxide synthase in mice with severe hypoxia-induced pulmonary hypertension. | Available online http content 2 5 306 Research article Upregulation of nitric oxide synthase in mice with severe hypoxia-induced pulmonary hypertension Karen A Fagan 1- Brian Morrissey Brian W Fouty 1 Koichi Sato1 Julie Wright Harral Kenneth G Morris Jr1 Marloes Hoedt-Miller Shanda Vidmar1 Ivan F McMurtry 1 and David M Rodman 1 Division of Pulmonary Sciences and Critical Care Medicine and Cardiovascular Pulmonary Research Laboratory University of Colorado Health Sciences Center Denver Colorado USA Correspondence Karen A Fagan MD 4200 East Ninth Avenue B-133 Denver CO 80262 USA. Tel 1 303 315 1305 fax 1 303 315 4871 e-mail Received 23 January 2001 Revisions requested 12 February 2001 Revisions received 9 July 2001 Accepted 2 August 2001 Published 4 September 2001 Respir Res 2001 2 306-313 2001 Fagan et al licensee BioMed Central Ltd Print ISSN 1465-9921 Online ISSN 1465-993X Abstract Background The importance of nitric oxide NO in hypoxic pulmonary hypertension has been demonstrated using nitric oxide synthase NOS knockout mice. In that model NO from endothelial NOS eNOS plays a central role in modulating pulmonary vascular tone and attenuating hypoxic pulmonary hypertension. However the normal regulation of NOS expression in mice following hypoxia is uncertain. Because genetically engineered mice are often utilized in studies of NO we conducted the present study to determine how hypoxia alters NOS expression in wild-type mice. Method Mice were exposed to sea level ambient conditions 5280 feet or severe altitude 17 000 feet for 6 weeks from birth and hemodynamics and lung NOS expression were assessed. Results Hypoxic mice developed severe pulmonary hypertension right ventricular systolic pressure RVsP 60 mmHg as compared with normoxic mice 27 mmHg . Using quantitative reversetranscription PCR it was found that expressions of eNOS and inducible NOS iNOS increased and respectively in the lung. In .
