Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Effect of hemofiltration filter adsorption on circulating IL-6 levels in septic rats. | Available online http content 6 5 429 Research Effect of hemofiltration filter adsorption on circulating IL-6 levels in septic rats John A Kellum1 and Michael K Dishart2 Associate Professor of Critical Care Medicine and Medicine Department of Critical Care Medicine University of Pittsburgh Medical Center PA USA 2Section Head Critical Care Department of Anesthesiology Western Pennsylvania Hospital Pittsburgh PA USA Correspondence John A Kellum Kellumja@ Received 9 January 2002 Revisions requested 17 February 2002 Revisions received 23 May 2002 Accepted 27 May 2002 Published 19 June 2002 Critical Care 2002 6 429-433 This article is online at http content 6 5 429 2002 Kellum and Dishart licensee BioMed Central Ltd Print ISSN 1364-8535 Online ISSN 1466-609X Abstract Introduction Hemofiltration may modulate the inflammatory response in sepsis through a variety of mechanisms. We sought to distinguish clearance from adsorption as the principal mechanism responsible for reducing circulating IL-6 levels with hemofiltration. Materials and methods Nine hours after cecal ligation and puncture in 18 adult male Sprague-Dawley rats we divided the rats into three groups 6 animals each and placed groups 2 and 3 on a hemofiltration circuit connected between the right carotid artery and femoral vein using an AN69 membrane. In the hemofiltration group group 2 ultrafiltrate was replaced with lactated Ringer s solution in the recirculation group group 3 the ultrafiltrate was reinfused into the animal. A sham group group 1 had an arteriovenous circuit inserted but no hemofiltration. Blood was obtained for measurement of IL-6 and tumor necrosis factor TNF at the start of hemofiltration and after 5 and 11 hours of treatment. Results and discussion IL-6 levels increased only in the sham-treated animals at baseline to pg ml at 11 hours P differences between groups 1 and 2 P and groups 1 and 3 P . TNF levels were highly
