Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"Platelets stimulate fibroblast-mediated contraction of collagen gels | Respiratory Research BioMed Central Research Open Access Platelets stimulate fibroblast-mediated contraction of collagen gels Ulrika Zagai 1 Karin Fredriksson1 Stephen I Rennard2 Joachim Lundahl1 and C Magnus Skold1 Address Department of Medicine Karolinska Hospital Stockholm Sweden and 2Department of Internal Medicine University of Nebraska Medical Center Omaha NE USA Email UlrikaZagai - Karin Fredriksson - Stephen I Rennard - Joachim Lundahl - C Magnus Skold - Corresponding author Published 17 October 2003 Received 29 May 2003 Accepted 17 October 2003 Respiratory Research 2003 4 13 This article is available from http content 4 l l 3 2003 Zagai et al licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Background Platelets are thought to play a role in a variety of inflammatory conditions in the lung some of which may lead to fibrosis. In the current study we tested the hypothesis that whole platelets and platelet lysate can mediate remodelling of extracellular matrix in vitro by affecting fibroblast-mediated contraction of a collagen gel. We also sought to determine to what extent platelet-derived growth factor PDGF and transforming growth factor-P TGF-P contribute to this effect. Methods Washed platelets isolated from healthy blood donors and platelet lysate freezing and thawing were cast together with human lung fibroblasts in three-dimensional collagen gels. The gels were then released and cultured for four days. PDGF and TGF-Pl concentrations were measured in culture supernatants by ELISA. Results Both platelets and platelet lysate augmented fibroblast-mediated gel contraction in a time and concentration dependent manner mean SEM of initial .
