Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Protection of pulmonary epithelial cells from oxidative stress by hMYH adenine glycosylase. | Respiratory Research BioMed Central Research Open Access Protection of pulmonary epithelial cells from oxidative stress by hMYH adenine glycosylase Ted M Kremer Mikael L Rinne Yi Xu Xian Ming Chen and Mark R Kelley Address Department of Pediatrics Herman B Wells Center for Pediatric Research Indiana University School of Medicine Indianapolis Indiana USA Email Ted M Kremer - kremert@ Mikael L Rinne - mrinne@ Yi Xu - yxu1@ Xian Ming Chen - xmchen@ Mark R Kelley - mkelley@ Corresponding author Published 27 September 2004 Received 23 April 2004 Accepted 27 September 2004 Respiratory Research 2004 5 16 doi 1465-9921-5-16 This article is available from http content 5 1 16 2004 Kremer et al licensee BioMed Central Ltd. This is an open-access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Oxygen toxicity is a major cause of lung injury. The base excision repair pathway is one of the most important cellular protection mechanisms that responds to oxidative DNA damage. Lesion-specific DNA repair enzymes include hOggl hMYH hNTH and hMTH. Methods The above lesion-specific DNA repair enzymes were expressed in human alveolar epithelial cells A549 using the retroviral vector. Cells were exposed to a 95 oxygen environment ionizing radiation IR or H2O2. Cell growth analysis was performed under non-toxic conditions. Western blot analysis was performed to verify over-expression and assess endogenous expression under toxic and non-toxic conditions. Statistical analysis was performed using the paired Student s t test with significance being accepted for p . Results Cell killing assays demonstrated cells over-expressing hMYH had improved survival to both increased oxygen and IR. Cell .
