Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:Inhibition of TNFalpha in vivo prevents hyperoxia-mediated activation of caspase 3 in type II cells. | Respiratory Research BioMed Central Research Open Access Inhibition of TNFalpha in vivo prevents hyperoxia-mediated activation of caspase 3 in type II cells Florian Guthmann 1 Heide Wissel1 Christian Schachtrup2 Angelika Tolle1 Mario Rudiger1 Friedrich Spener2 and Bernd Rustow 1 Address 1Humboldt-Universitat zu Berlin Klinik fur Neonatologie Charité Campus Mitte D-10098 Berlin Germany and 2Westfalische Wilhelms-Universitat Munster Institut fur Biochemie Wilhelm-Klemm-Str. 2 D-48149 Munster Germany Email Florian Guthmann - Heide Wissel - Christian Schachtrup - Angelika Tolle - Mario Rudiger - Friedrich Spener- spener@ Bernd Rustow - Corresponding author Published 21 January 2005 Received 19 August 2004 Respiratory Research 2005 6 10 doi l465-992l-6-l0 Accepted 21 January 2005 This article is available from http content 6 l l0 2005 Guthmann et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The mechanisms during the initial phase of oxygen toxicity leading to pulmonary tissue damage are incompletely known. Increase of tumour necrosis factor alpha TNFalpha represents one of the first pulmonary responses to hyperoxia. We hypothesised that in the initial phase of hyperoxia TNFalpha activates the caspase cascade in type II pneumocytes TIIcells . Methods Lung sections or freshly isolated TIIcells of control and hyperoxic treated rats 48 hrs were used for the determination of TNFalpha ELISA TNF-receptor l Western blot and activity of caspases 8 3 and 9 colorimetrically . NF-kappaB activation was .
