Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"Expression of the α7 nicotinic acetylcholine receptor in human lung cells. | Respiratory Research BioMed Central Research Open Access Expression of the a7 nicotinic acetylcholine receptor in human lung cells Howard K Plummer III 1 Madhu Dhar1 and Hildegard M Schuller2 Address 1Molecular Cancer Analysis Laboratory Department of Pathobiology College of Veterinary Medicine University of Tennessee Knoxville TN 37996-4542 USA and 2Experimental Oncology Laboratory Department of Pathobiology College of Veterinary Medicine University of Tennessee Knoxville TN 37996-4542 USA Email Howard K Plummer - hplummer@ Madhu Dhar - mdhar@ Hildegard M Schuller - hmsch@ Corresponding author Published 04 April 2005 Received 06 January 2005 Respiratory Research 2005 6 29 doi 1465-9921 -6-29 Accepted 04 April 2005 This article is available from http content 6 1 29 2005 Plummer et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background We and others have shown that one of the mechanisms of growth regulation of small cell lung cancer cell lines and cultured pulmonary neuroendocrine cells is by the binding of agonists to the a7 neuronal nicotinic acetylcholine receptor. In addition we have shown that the nicotine-derived carcinogenic nitrosamine 4 methylnitrosamino -1 - 3-pyridyl -l-butanone NNK is a high affinity agonist for the a7 nicotinic acetylcholine receptor. In the present study our goal was to determine the extent of a7 mRNA and protein expression in the human lung. Methods Experiments were done using reverse transcription polymerase chain reaction RT-PCR a nuclease protection assay and western blotting using membrane proteins. Results We detected mRNA for the neuronal nicotinic acetylcholine receptor a7 receptor in seven small cell lung cancer SCLC
