Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú y đề tài: Assessment of sperm quality traits in relation to fertility in boar semen. | Acta Veterinaria Scandinavica BioMed Central Research Open Access Assessment of sperm quality traits in relation to fertility in boar semen Neringa Sutkeviciene 1 Vita Riskeviciene1 Aloyzas Januskauskas1 Henrikas Zilinskas1 and Magnus Andersson2 Address Department of Non-infectious diseases Faculty of Veterinary Medicine Lithuanian Veterinary Academy Kaunas Lithuania and 2Department of Production Animal Medicine University of Helsinki Saarentaus Finland Email Neringa Sutkeviciene - nerija@ Vita Riskeviciene - vitarisk@ Aloyzas Januskauskas - janusalo@ Henrikas Zilinskas - hezil@ Magnus Andersson - Corresponding author Published 16 December 2009 Received 28 September 2009 . _ . r. J - . r I r Accepted 16 December 2009 Acta Veterinaria Scandinavica 2009 51 53 doi l75l-0l47-5l-53 This article is available from http content 5l l 53 2009 Sutkeviciene et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Several studies have been published where sperm plasma membrane integrity correlated to fertility. In this study we describe a simple fluorometer-based assay where we monitored the fluorescence intensity of artificially membrane-ruptured spermatozoa with a fixed time staining with fluorescent DNA dyes. Methods Membrane-impermeant fluorescent dyes Hoechst 33258 H258 and propidium iodide PI were used to measure the fluorescence of the nucleus in artificially membrane ruptured spermatozoa and membrane-permeant dye Hoechst 33342 H342 was used to measure fluorescence of intact spermatozoa. The concentration of spermatozoa in insemination doses varied from X l06 ml to 50 X l06 ml and the average value was 35 X l06 ml. Each boar .
