Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú yđề tài: Comparative study on the in vitro and in vivo properties of two bovine herpesvirus-5 reference strains. | Ladelfa et al. Acta Veterinaria Scandinavica 2011 53 37 http content 53 1 37 AVS ACTAVETERINARIA SCANDINAVICA RESEARCH Open Access Comparative study on the in vitro and in vivo properties of two bovine herpesvirus-5 reference strains 13 1 13 4 2 María F Ladelfa 1 María P Del Médico Zajac Fiorella Kotsias 1 Fernando Delgado Benoit Muylkens Julien Thiry2 Etienne Thiry2 and Sonia A Romera1 3 Abstract Background Bovine herpesvirus 5 BoHV-5 is an alphaherpesvirus responsible for meningoencephalitis in young cattle and it is antigenically and genetically related to bovine herpesvirus 1. BoHV-5 outbreaks are sporadic and restricted in their geographical distribution being mostly detected in the Southern hemisphere. The N569 and A663 strains are prototypes of the a and b subtypes of BoHV-5 however scarce information about their in vitro and in vivo properties is currently available. Methods For the in vitro comparison between BoHV-5 A663 and N569 strains viral growth kinetics lysis and infection plaque size assays were performed. Additionally an experimental infection of cattle with BoHV-5 A663 and N569 strains was carried out. Viral excretion development of neurological signs presence of specific antibodies in serum and nasal swabs and presence of latent BoHV-5 DNA in trigeminal ganglion were analyzed. Histopathological examination of samples belonging to inoculated animals was also performed. Results The lytic capacity and the cell-to-cell spread was lower for the A663 strain compared to the N569 strain however the production of total infectious viral particles was similar between both strains. Concerning the in vivo properties the A663 and N569 strains are able to induce similar degrees of pathogenicity in cattle. Conclusions Our results show that the A663 strain used in this study is less adapted to in vitro replication in MDBK cells than the N569 strain and although slight differences were observed both strains are able to induce a similar degree
