Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Retrovirology cung cấp cho các bạn kiến thức về ngành y đề tài: " Enhanced macrophage tropism of HIV in brain and lymphoid tissues is associated with sensitivity to the broadly neutralizing CD4 binding site antibody b12. | Retrovirology BioMed Central Open Access Short report Enhanced macrophage tropism of HIV in brain and lymphoid tissues is associated with sensitivity to the broadly neutralizing CD4 binding site antibody b12 Rebecca L Dunfee1 2 Elaine R Thomas1 2 and Dana Gabuzda 1 3 Address Department of Cancer Immunology and AIDS Dana Farber Cancer Institute Boston MA USA 2Department of Pathology Harvard Medical School Boston MA USA and 3Department of Neurology Harvard Medical School Boston MA USA Email Rebecca L Dunfee - dunfeer@ Elaine RThomas - EThomas@ Dana Gabuzda - dana_gabuzda@ Corresponding author Published 20 July 2009 Received 20 April 2009 Retrovirology 2009 6 69 doi 1742-4690-6-69 Accepted 20 July 2009 This article is available from http content 6 1 69 2009 Dunfee et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Macrophages in the central nervous system CNS and other tissues are an important cellular reservoir for human immunodeficiency virus type I HIV infection particularly in the later stages of disease. Macrophage-tropic HIV strains have an enhanced capacity to enter cells expressing low levels of CD4 through mechanisms that are not well understood. Here we use a panel of primary HIV envelopes from brain and lymphoid tissues to examine the relationship between neutralization sensitivity to reagents targeting the CD4 binding site and virus entry into macrophages. Neutralization assays using pseudotyped viruses showed an association between the capacity of HIV to enter macrophages and increased sensitivity to the broadly neutralizing monoclonal antibody mAb b12 which recognizes a conserved epitope overlapping the CD4 binding site but not .
