Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Retrovirology cung cấp cho các bạn kiến thức về ngành y đề tài: Visualizing fusion of pseudotyped HIV-1 particles in real time by live cell microscopy. | Retrovirology BioMed Central Research Visualizing fusion of pseudotyped HIV-I particles in real time by live cell microscopy Peter Koch1 Marko Lampe1 3 William J Godinez2 Barbara Muller1 Karl Rohr2 Hans-Georg Krausslich 1 and Maik J Lehmann1 Address Department of Virology Universitatsklinikum Heidelberg Im Neuenheimer Feld 324 69120 Heidelberg Germany 2Department of Bioinformatics and Functional Genomics BIOQUANT IPMB University of Heidelberg Im Neuenheimer Feld 267 69120 Heidelberg Germany and 3Division of Cell Biology MRC Laboratory of Molecular Biology Hills Road Cambridge CB20QH UK Email Peter Koch - Marko Lampe - mlampe@ William J Godinez - Barbara Muller - barbara_mueller@ Karl Rohr - Hans-Georg Krausslich - Maik J Lehmann - Corresponding author Open Access Published 18 September 2009 Received 22 April 2009 Accepted 18 September 2009 Retrovirology 2009 6 84 doi 1742-4690-6-84 This article is available from http content 6 1 84 2009 Koch et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Most retroviruses enter their host cells by fusing the viral envelope with the plasma membrane. Although the protein machinery promoting fusion has been characterized extensively the dynamics of the process are largely unknown. Results We generated human immunodeficiency virus-1 HIV-1 particles pseudotyped with the envelope Env protein of ecotropic murine leukemia virus eMLV to study retrovirus entry at the plasma membrane using live-cell microscopy. This Env protein mediates highly .
