Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Retrovirology cung cấp cho các bạn kiến thức về ngành y đề tài: Cell-specific targeting of lentiviral vectors mediated by fusion proteins derived from Sindbis virus, vesicula. | Zhang et al. Retrovirology 2010 7 3 http content 7 1 3 RETR0VIR0L0GY RESEARCH Open Access Cell-specific targeting of lentiviral vectors mediated by fusion proteins derived from Sindbis virus vesicular stomatitis virus or avian sarcoma leukosis virus 1 4 5 Xian-Yang Zhang 1 Robert H Kutner Agnieszka Bialkowska 1 Michael P Marino William B Klimstra Jakob Reiser1 4 Abstract Background The ability to efficiently and selectively target gene delivery vectors to specific cell types in vitro and in vivo remains one of the formidable challenges in gene therapy. We pursued two different strategies to target lentiviral vector delivery to specific cell types. In one of the strategies vector particles bearing a membrane-bound stem cell factor sequence plus a separate fusion protein based either on Sindbis virus strain TR339 glycoproteins or the vesicular stomatitis virus G glycoprotein were used to selectively transduce cells expressing the corresponding stem cell factor receptor c-kit . An alternative approach involved soluble avian sarcoma leukosis virus receptors fused to cell-specific ligands including stem cell factor and erythropoietin for targeting lentiviral vectors pseudotyped with avian sarcoma leukosis virus envelope proteins to cells that express the corresponding receptors. Results The titers of unconcentrated vector particles bearing Sindbis virus strain TR339 or vesicular stomatitis virus G fusion proteins plus stem cell factor in the context of c-kit expressing cells were up to X 105 transducing units per ml while vector particles lacking the stem cell factor ligand displayed titers that were approximately 80 fold lower. On cells that lacked the c-kit receptor the titers of stem cell factor-containing vectors were approximately 40 times lower compared to c-kit-expressing cells. Lentiviral vectors pseudotyped with avian sarcoma leukosis virus subgroup A or B envelope proteins and bearing bi-functional bridge proteins encoding .
