Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Retrovirology cung cấp cho các bạn kiến thức về ngành y đề tài: Experimental depletion of CD8+ cells in acutely SIVagm-Infected African Green Monkeys results in increased viral replication. | Gaufin et al. Retrovirology 2010 7 42 http content 7 1 42 gtr RETR0VIR0L0GY RESEARCH Open Access Experimental depletion of CD8 cells in acutely SIVagm-Infected African Green Monkeys results in increased viral replication Thaidra Gaufin1 Ruy M Ribeiro2 Rajeev Gautam1 Jason Dufour3 Daniel Mandell1 Cristian Apetrei4 and Ivona Pandrea 5 Abstract Background In vivo CD8 cell depletions in pathogenic SIV infections identified a key role for cellular immunity in controlling viral load VL and disease progression. However similar studies gave discordant results in chronically-infected SMs leading some authors to propose that in natural hosts SIV replication is independent of cellular immunity. To assess the role of cellular immune responses in the control of SIV replication in natural hosts we investigated the impact of CD8 cell depletion during acute SIV infection in AGMs. Results Nine AGMs were infected with and were followed up to day 225 . Four were intravenously infused with the cM-T807 antibody on days 0 50 mg kg 6 and 13 10 mg kg respectively post infection . . CD8 cells were depleted for up to 28 days . in peripheral blood and LNs in all treated AGMs. Partial CD8 T cell depletion occurred in the intestine. SIVagm VLs peaked at similar levels in both groups 107-108 RNA copies ml . However while VLs were controlled in undepleted AGMs reaching set-point levels 104-105 RNA copies ml by day 28 . high VLs 106 RNA copies ml were maintained by day 21 . in CD8-depleted AGMs. By day 42 . VLs were comparable between the two groups. The levels of immune activation and proliferation remained elevated up to day 72 . in CD8-depleted AGMs and returned to preinfection levels in controls by day 28 . None of the CD8-depleted animals progressed to AIDS. Conclusion CD8 cells are responsible for a partial control of postacute viral replication in AGMs. In contrast to macaques the SIVagm-infected AGMs are able to
