Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Higher levels of Zidovudine resistant HIV in the colon compared to blood and other gastrointestinal compartments in HIV infectio. | van Marle et al. Retrovirology 2010 7 74 http content 7 1 74 RETR0VIR0L0GY RESEARCH Open Access Higher levels of Zidovudine resistant HIV in the colon compared to blood and other gastrointestinal compartments in HIV infection Guido van Marle Deirdre L Church Kali D Nunweiler Kris Cannon Mark A Wainberg M John Gill Abstract Background The gut-associated lymphoid tissue GALT is the largest lymphoid organ infected by human immunodeficiency virus type 1 HIV-1 . It serves as a viral reservoir and host-pathogen interface in infection. This study examined whether different parts of the gut and peripheral blood lymphocytes PBL contain different drugresistant HIV-1 variants. Methods Gut biopsies esophagus stomach duodenum and colon and PBL were obtained from 8 HIV-1 infected preHAART highly active antiretroviral therapy patients at three visits over 18 months. Patients received AZT ddI or combinations of AZT ddI. HIV-1 Reverse transcriptase RT -coding sequences were amplified from viral DNA obtained from gut tissues and PBL using nested PCR. The PCR fragments were cloned and sequenced. The resulting sequences were subjected to phylogenetic analyses and antiretroviral drug mutations were identified. Results Phylogenetic and drug mutation analyses revealed differential distribution of drug resistant mutations in the gut within patients. The level of drug-resistance conferred by the RT sequences was significantly different between different gut tissues and PBL and varied with antiretroviral therapy. The sequences conferring the highest level of drug-resistance to AZT were found in the colon. Conclusion This study confirms that different drug-resistant HIV-1 variants are present in different gut tissues and it is the first report to document that particular gut tissues may select for drug resistant HIV-1 variants. Introduction Science has been confronted with the problem of drugresistance virtually since the introduction of the first antiretroviral drugs .
