Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: HIV-1 subtype C envelope characteristics associated with divergent rates of chronic disease progression. | Archary et al. Retrovirology 2010 7 92 http content 7 1 92 RETR0VIR0L0GY RESEARCH Open Access HIV-1 subtype C envelope characteristics associated with divergent rates of chronic disease progression 1 1 1 1 Derseree Archary Michelle L Gordon Taryn N Green Hoosen M Coovadia Philip JR Goulder Thumbi Ndung u1 Abstract Background HIV-1 envelope diversity remains a significant challenge for the development of an efficacious vaccine. The evolutionary forces that shape the diversity of envelope are incompletely understood. HIV-1 subtype C envelope in particular shows significant differences and unique characteristics compared to its subtype B counterpart. Here we applied the single genome sequencing strategy of plasma derived virus from a cohort of therapy naive chronically infected individuals in order to study diversity divergence patterns and envelope characteristics across the entire HIV-1 subtype C gp160 in 4 slow progressors and 4 progressors over an average of months. Results Sequence analysis indicated that intra-patient nucleotide diversity within the entire envelope was higher in slow progressors but did not reach statistical significance p . However intra-patient nucleotide diversity was significantly higher in slow progressors compared to progressors in the C2 p V3 p and C3 p regions. Increased amino acid length and fewer potential N-linked glycosylation sites PNGs were observed in the V1-V4 in slow progressors compared to progressors p and p respectively . Similarly gp41 in the progressors was significantly longer and had fewer PNGs compared to slow progressors p and p respectively . Positive selection hotspots mapped mainly to V1 C3 V4 C4 and gp41 in slow progressors whereas hotspots mapped mainly to gp41 in progressors. Signature consensus sequence differences between the groups occurred mainly in gp41. Conclusions These data suggest that separate regions of envelope are under .
