Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: CD4+CD25+ T regulatory cells from FIV+ cats induce a unique anergic profile in CD8+ lymphocyte targets. | Fogle et al. Retrovirology 2010 7 97 http content 7 1 97 RETROVIROLOGY RESEARCH Open Access CD4 CD25 T regulatory cells from FIV cats induce a unique anergic profile in CD8 lymphocyte targets Jonathan E Fogle Wayne A Tompkins Mary B Tompkins Abstract Background Using the FIV model we reported previously that CD4 CD25 T regulatory Treg cells from FIV cats are constitutively activated and suppress CD4 CD25- and CD8 T cell immune responses. In an effort to further explore Treg-mediated suppression we asked whether Treg cells induce anergy through the alteration of production of cyclins cyclin-dependent kinases and their inhibitors. Results Lymphocytes were obtained from control or FIV cats and sorted by FACS into CD4 CD25 and CD8 populations. Following co-culture with CD4 CD25 cells CD8 targets were examined by Western blot for changes in cyclins D3 E and A retinoblastoma Rb protein as well as the cyclin dependent kinase inhibitor p21cip1. Following co-culture with CD4 CD25 cells we observed up-regulation of p21cip1 and cyclin E with downregulation of cyclin D3 in CD8 cells from FIV cats. As expected CD8 targets from control cats were quiescent with little up-regulation of p21cip1 and cyclin E. There was also a lack of Rb phosphorylation in CD8 targets consistent with late G1 cell cycle arrest. Further IL-2 mRNA was down regulated in CD8 cells after co-culture with CD4 CD25 Treg cells. Following CD4 CD25 co-culture CD8 targets from FIV cats also had increased Foxp3 mRNA expression however these CD8 Foxp3 cells did not exhibit suppressor function. Conclusions Collectively these data suggest that CD4 CD25 Treg cells from FIV cats induce CD8 anergy by disruption of normal G1 to S cell cycle progression. Background Using FIV as an AIDS lentivirus model we reported previously that CD4 CD25 Treg cells in both the acute phase and long-term asymptomatic phase of infection are constitutively activated and suppress CD4 CD25- and CD8 T cell immune responses
